fdhF promoter

来源: E.coli BL21(DE3)
功能: promoter

Summary

An oxygen-responsive promoter

基本信息

定量方法 Flow cytometry. 20,000 cells was collected within a small forward and side scatter gate to reduce extrinsic noise.
培养条件 Strian grow in LB media to the OD600 of 1.0 in 5 L bioreactor,and then adjust the dissolved oxygen to 20 % to induce expression.
报告基因 GFP
诱导剂 Dissolved oxygen
提交人 Wang Yong

元件序列

元件名 序列 序列长度 定量结果
fdhF
GCGGGTATCAGGCAGGTCCCGTCGTCGAGAAAAAATAAAATGTCTGCCGCGTGATGGCTGTCACGCGGTATTTCGTTTCGTCACGTCAAAACTGACGACAGCCTGTTTTTCGTCAGAGTTTTGAATAAATAGTGCCCGTAATATCAGGGAATGACCCCACATAAAATGTGGCATAAAAGATGCATACTGTAGTCGAGAGCGCGTATGCGTGATTTGATTAACTGGAGCGAGACCGATGAAAAAAGTCGTCACGGTTTGCCCCTATTG
267 112.34±11.26 AU (after 6 h induction)/5.43±1.63 AU(before induction)