pcad promoter

来源: E.coli BL21(DE3)
功能: promoter

Summary

A pH responsive-promoter

基本信息

定量方法 Flow cytometry. 20,000 cells was collected within a small forward and side scatter gate to reduce extrinsic noise.
培养条件 Strian grow in LB media to the OD600 of 1.0 in 5 L bioreactor,and then adjust the pH to 5.5 using 1 M HCl to induce expression.
报告基因 GFP
诱导剂 pH value of media
提交人 Wang Yong

元件序列

元件名 序列 序列长度 定量结果
pcad
cacgcaatccattgtaaacattaaatgtttatcttttcatgatatcaacttgcgatcctgatgtgttaataaaaaacctcaagttctcacttacagaaacttttgtgttatttcacctaatctttaggattaatccttttttcgtgagtaatcttatcgcc
161 200.45±12.65AU (after 6 h induction)/15.94±2.43 AU(with pH of 8.0)