pJA-sll0039 -Final

来源: Synechocystis sp. PCC6803
功能: two-component response regulator

Summary

sll0039 encodes a two-component response regultor Sll0039 invovling the regulation of 1-butanol tolerance. In this part, sll0039 was ligated into a shuttle vetor pJA2 under a light-inducible promoter PpsbA2.

基本信息

定量方法 Growth patterns. For measurements of growth patterns, 5 mL fresh cells at OD630 of 0.2 were collected by centrifugation at 3000 x g and 4 oC and were then inoculated into 25 mL of BG11 liquid medium in a 100 mL flask; 1-butanol treatment was carried out by adding 0.20% (v/v) 1-butanol (Merck, USA) to the medium at beginning of the cultivation, each with three replicates. Cell density was measured on an ELx808 Absorbance Microplate Reader (BioTek, Winooski, VT, USA) at OD630.
培养条件 Synechocystis sp. PCC 6803 were grown in aBG11 medium under a light intensity of approximately 50 μmol photons m-2s-1
报告基因 N/A
诱导剂 Light
提交人 Tao Sun

元件序列

元件名 序列 序列长度 定量结果
Sll0039
ATGGGCAGCGCACTTGTTATTGACGATTCCTCTACAGAACGTTCAATTATCAGTGATTTTTGTCAGAAACTAGGCATTAACGTCACCACCGCCATCAGTGGAGAAGAAGCCCTGGAAAAACTTTCCCAGGCAGTGCCGGACGTAATTATCTTAGATATTGTGCTGCCGGGGCGCAGTGGTTTTGAAATTTGTCGAGAATTGAAAGACAAAGACCGGACTAAAAGTATTCCAATAATCCTCTGTTCCACTAAGGCCACGGATATGGATAAATTTTGGGGCAAACGCCAAGGGGCGGACGCCTATATTACTAAACCCATTGACCAAGAGGAGTTCAACACGGTAATCAAGCAATTTATCTGA
360 OD630 of Wide type could reach about 0.25 at 72 h while the Sll0039-overexpressing strain could reach about 0.33.