promoters of Streptomyces from PNAS-2015-Bai

来源: Streptomyces venezuelae ISP5230
功能: Promoter

Summary

A group native or synthetic promoters of Streptomyces

基本信息

定量方法 Protoplasts was prepared from mycelia of Streptomyces at first and then the protoplasts were analyzed by BD FACSCalibur Flow Cytometer with a 488-nmexcitation laser and the FL1 (530/30 nm band-pass filter) detector. Eachsample collected 50,000 events, and the data were further acquired using BDFACSuite software and analyzed by FlowJo 9.3.2 software (Tree Star, Inc.). The parameters of the FACS setting were as follows: FSC-E00, SSC-650, FL1-400, FL2-400; threshold: FSC-50, SSC-400. All date compared with the date of strong promoter kasOp*
培养条件 Mannitol soya flour medium was used for conjugation, and malt extract–yeast extract–maltose medium was used for liquid inoculation of the spores of S. venezuelae ISP5230. The media were supplemented with different antibiotics at appropriate concentrations as follows: ampicillin at 100 μg/mL, apramycin at 50 μg/mL, nalidixic acid at 25 μg/mL, kanamycin at 25 μg/mL, and chloramphenicol at 25 μg/mL Spores of S. avermitilis WT transformants were used to inoculate a 250-mL flask containing 25 mL seed medium [glucose (5 g), soy flour (15 g), and yeast extract (5 g) per liter, at pH 7.2], and the culture was allowed to grow with shaking (220 rpm) at 30 °C for 2 d. A 1-mL aliquot of the culture was used to inoculate a 250-mL flask containing 50 mL production medium, CaCO3 (5 g), and yeast extract (2 g) per liter at pH 7.0] (41). The samples were incubated with shaking (220 rpm) at 28 °C for 5 d.
报告基因 GFP
诱导剂
提交人 Yan Xing;Zhao Wenfang

元件序列

元件名 序列 序列长度
SP22
tgttcacattcgaaccgtctctgctttgacaagtcgcaaaacaacctgtaaagtcgtggcca
62
SP11
tgttcacattcgaaccgtctctgctttgacaatggcttgaattggggtgtaaagtcgtggcca
63
P7
tccgttcccggcggggagcgggacacccggggagttcgcccggaaatgtccgaagctaggcgaagaaagtgtgacgagcacgaagtccctgctccctgccggtgcgaatgtgccacgcgatagggtgtggtgacaggcacac
142
SP42
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcgggtgaa
63
SP16
tgttcacattcgaaccgtctctgctttgacatacttgggtcgggcactgtaaagtcgtggcca
63
SP33
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcgatggca
63
SP15
tgttcacattcgaaccgtctctgctttgacacctacgtgacacatcttgtaaagtcgtggcca
63
SP18
tgttcacattcgaaccgtctctgctttgacaggctctgctgtgaacgtgtaaagtcgtggcca
63
SP23
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcctgctaa
63
SP44
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtctggtgta
63
SP41
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtccagctga
63
SP8
tgttcacattcgaaccgtctctgctttgacacccgcggattacctcctgtaaagtcgtggcca
63
SP9
tgttcacattcgaaccgtctctgctttgacacgcaagatcaacccgctgtaaagtcgtggcca
63
SP19
tgttcacattcgaaccgtctctgctttgacacgacccactttcgctgtgtaaagtcgtggcca
63
P1
ctcggcggcgagcgggggacgttggtcccgcacccgccgggacccgggtcccggcgggcgaaacggaggtccgtgctcctactggcgagtacggatctccggccgtatccggtggcgtgcggtggccctctcgatgtcacccccggggcctcggagaggtagggtcggaagcggtcggggacatccctatacaactcgccggcgtcgaaaaccggcgta
219
P2
gatccccaagggctcagggccttggcatgatccgctcagtgatgctagtgcggtccatttgttttgaccgcagcgtatgaggtaggtacgctcagaccttgtgcctggggtgtgccctggctctcgtgcgtgccttcaaccgcacaaggggagccgtcaccggccaccgtaatctgcgcccttttgcttcacggcgagagtctgcagcattcgacacacccgaccgcgtgggtcggcaatgttccaggttagcttcaccattcggc
266
P4
aggggcgcgcggccccggccgccggccgtcccgtccgcgggcccgtggccggggcggtcccggcgtgtcgcggcggacagcatttgttttgacccagctccgtgaggtaggtacgctcaagccttgtgcctggggtgtgcctgggctcgggtgcgtgtcctcaaccgcatcgcgagtccgtcagtagccaccgcaatctgcgcccttcctgccttcggggcgggagtccgcagtattcgacacacccgaccgcgtgggtcggcgatgttccaggttagtttcacgaacggc
291
P3
tgttcacattcgaaccgtctctgctttgacaaaccggtgtgctggtttgtaaagtcgtggccaggagaatacgacag
78
SP27
tgttcacattcgaaccgtctctgctttgacaaggtcccggtcttaggtgtaaagtcgtggcca
63
SP12
tgttcacattcgaaccgtctctgctttgacaggtccatacacgcgcttgtaaagtcgtggcca
63
SP36
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtctaaccga
63
P9
cggagagtagtggggacaaaggtcccatggcggcccgtacccgttggtaggtacgggccgtcggccgttttccctcactttccgggctcgtacggatgcgctcgatgtcactcccgggcgcggggggaggtagggtcgtaagcggtcggggacatcccaaaaaacaaaaatctcgccggcgtcgagcacaccggcgta
198
SP30
tgttcacattcgaaccgtctctgctttgacatcgtgtggcgcttgggtgtaaagtcgtggcca
63
SP25
tgttcacattcgaaccgtctctgctttgacagaggtaggcacgctcatgtaaagtcgtggcca
63
SP21
tgttcacattcgaaccgtctctgctttgacaatgggtccgcctcgagtgtaaagtcgtggcca
63
SP5
tgttcacattcgaaccgtctctgctttgacatcttatgattctcgactgtaaagtcgtggcca
63
P15
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcgtggcca
64
P14
cccgccgcgggcgctggaggctcgggcgggccccgggccggaggcggccgcgaccacgacgcccgcgggacgtgacgagcggcacgactcgacgactccgggctcctttgacgctgtccgtcgcgccgggtagcgtaggacaccgtgcccgcgccgtcgggccctcgcgcgtgcactcggtcgaccgctccctgccggagtgggtgcgggtgcacggggtggctccccacctcctctcggatcggtcctcgcggactgccgccgtgcggaggaccggggcgacacgcccgggcgcgggggtcggtgcgggactccagacctccggggtagtcgtgcgacgggcgacgatccgggccgagccggccgtcctgggtgacgggtgccggtcagaccagagaa
399
SP06
tgttcacattcgaaccgtctctgctttgacatggtctagccgttgattgtaaagtcgtggcca
63
SP43
tgttcacattcgaaccgtctctgctttgacacggacaagcgctatggtgtaaagtcgtggcca
63
SP7
tgttcacattcgaaccgtctctgctttgacaattggattcacgtatgtgtaaagtcgtggcca
63
SP10
tgttcacattcgaaccgtctctgctttgacatgttcttacggtcacatgtaaagtcgtggcca
63
SP35
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcgggggga
63
SP17
tgttcacattcgaaccgtctctgctttgacaatcgcagactttatgctgtaaagtcgtggcca
63
P8
gagcgagtgtccgttcgagtggcggcttgcgcccgatgctagtcgcggttgatcggcgatcgcaggtgcacgcggtcgatcttgacggctggcgagaggtgcggggaggatctgaccgacgcggtccacacgtggcaccgcgatgctgttgtgggcacaaccgtgccg
169
SP14
tgttcacattcgaaccgtctctgctttgacacgacccactttcgctgtgtaaagtcgtggcca
63
SP4
tgttcacattcgaaccgtctctgctttgacagccttgctgaaaatcttgtaaagtcgtggcca
63
SP38
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcgctggca
63
P11
cacgggccggtgaaccccgcatgaatgttggttcatgtggggtttgcgccagctgtgtcgcagttgccccccgccactgttcccggggcatgaatcccgctagaatgaacacggaagagcaggtggaccccgggtcgtcaccgcacttcgggtgtgggatttatgccgattggttttgatcccacatccggacgggccggagtgcaccgcacacc
215
SP37
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcatccgta
63
SP13
tgttcacattcgaaccgtctctgctttgacaagccgacctataaacatgtaaagtcgtggcca
63
SP39
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcccatgaa
63
SP34
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcttcaaga
63
P10
ctccggtcggtcaacggcagttggtgtcgtgtaaccgaaacgagtggtcaggcctcaaggacggggcggtccgtacccctactggtgggtacggaccgcctcgccgtgtatggggcatctcgatgtcaccacaggggcttcagggaggtagggtcgtaggcggtcggggacatcccatacaactcgccggcgtcgaatgccggcgta
207
SP28
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcaaggaga
63
P13
gctgctccttcggtcggacgtgcgtctacgggcaccttaccgcagccgtcggctgtgcgacacggacggatcgggcgaactggccgatgctgggagaagcgcgctgctgtacggcgcgcaccgggtgcggagcccctcggcgagcggtgtgaaacttctgtgaatggcctgttcggttgctttttttatacggctgccagataaggcttgcagcatctgggcggctaccgctatgatcggggcgttcctgcaattcttagtgcgag
266
SP29
tgttcacattcgaaccgtctctgctttgacatcctacacacgtttagtgtaaagtcgtggcca
63
P5
tcgtgaccggtccggctgcgtggtctgagtttgcagccagagtgtctggtcagagcgtgaggtcactatcaccgtggcctcgctttgacctcgtggcgcgccgacaggtatcgttatgggtcgtgtccggcatgcccgggccgatctgtgtgcctagctaggtattgccgtgcgcgcgttcgagcagaaactgctgatgaacgagcatccggctgcctgtgtgggggtatgcgacacgcccgacctcgggggcagtgcgcggcatgaaagtgctgagtacagactctgaggtgggtgaggagcagcagctctcttgctccaactgctagatccctaatttcagaacagccacgttttcgaacggcccgagttcgctcgagcagtctgtt
389
SP20
tgttcacattcgaaccgtctctgctttgacacatgacgctcacccgttgtaaagtcgtggcca
63
SP2
tgttcacattcgaaccgtctctgctttgacagagcccctaccagaattgtaaagtcgtggcca
63
SP24
tgttcacattcgaaccgtctctgctttgacaaccaaggcacatctaatgtaaagtcgtggcca
63
SP1
tgttcacattcgaaccgtctctgctttgacatggagagaagttttgtaaagtcgtggcca
60
SP3
tgttcacattcgaaccgtctctgctttgacaatgatttaagcgtaaatgtaaagtcgtggcca
63
SP40
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcccgtaga
63
SP32
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtccagtgta
63
SP26
tgttcacattcgaaccgtctctgctttgacagctcactgggcatgggtgtaaagtcgtggcca
63
P12
aaccgggtccgcgatcggcggaggcgaacacttttgaccactatgagttcatccaggtaagcttggtcgccgtgcctggtgaatgccaggacgatcgctcgtgcccatactgcaggccggacctccgggaccacgtacggacacgcgacacgcccgacctcggggtacgtgcgaggcgggtagctacttcccggaaacgggactgaccagcagagacggcgaaagccggaactgccggtggggccgcgctgcggaagtcgcaccctcgatgagggacggcttgcccggagctacaaccgcagcagtagagccaccggccggacggccgatggcagccgaaacga
344
P6
cctgccctccgtcagggtgggctgcgcacgttcgggccacgcgcattagggtatgagtcacatcgaccgatgaaccccacgtccgcgtcgtgcacacacggattcggagtatgcgtgggtcaagttgcacaccagaat
138
SP31
tgttcacattcgaaccgtctctgctttgacaacatgctgtgcggtgttgtaaagtcagtaaca
63